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Application examples

Description:
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Information

Everolimus

Chromatographic column:50DAC
Packing:330 g C18(10 mm)
Sample loading capacity:0.1%
Yield:> 90%
Mobile phase:Acetonitrile/H₂O(68:32)
Purity:> 99.8% (Single impurity<0.1%)

Everolimus Reverse phase removes mainly isomers

Chromatographic column:500DAC
Packing:30 kg Silica gel(7 mm)
Sample loading capacity:1.5%
Mobile phase:n-hexane-ethyl acetate
Yield> 85%
Purity:> 97.5% 

Everolimus Normal phase purification removes both sirolimus and isomers

 

Vitamin K1

Chromatographic column:200DAC
Packing:5 kg C18(10 mm)
Sample loading capacity:2%
Yield:>90%
Mobile phase:100% Methanol
Purity:> 99.8% (Single impurity<0.1%)

Vitamin K1 Reverse phase removes mainly impurities other than isomers

Chromatographic column:100DAC
Packing:1.2 kg Silica gel(10 mm)
Sample loading capacity:3%
Yield:> 80%
Isomers:<13% (Original sample>23%)
Mobile phase:n-hexane-ethyl acetate

Vitamin K1 Normal phase mainly removes isomers

 

Application examples of reversed-phase chromatography packing

       The major impurity of a certain biological drug with a relative retention of 0.95 was difficult to be separated on a common C8 packing with a serious spreading of the peak shape of the main peak preparation. Upon packing optimization, it was found that with the increase of bonded phase density, the peak shape of the main peak preparation was narrowed and the separation from the major impurities was improved. Finally, the product was purified for industrial separation on optimized C8 packing. 

 

Acchrom has advanced packing synthesis technology, which can design and adjust the packing to satisfy the specific separation requirements of the project, so as to optimize the separation effect to the greatest extent. 

 

Application examples of normal phase chromatography packing

Flurbiprofen ester A neuromuscular blocking agent

Chromatographic column:600DAC
Packing:45 kg Silica gel(10 mm)
Sample loading capacity:8%
Mobile phase:n-hexane-ethyl acetate
Yield:> 95%
Purity:> 99.5% (Single impurity<0.1%)

Synthetic sample flurbiprofen ester has achieved separation with high loading capacity, high efficiency and high quality 

Chromatographic column:600DAC
Packing:45 kg Diol(10 mm)
Sample loading capacity:0.5%
Yield:> 90%
Purity:> 99.5% (Single impurity<0.2%)
Mobile phase:Dichloromethane-methanol

Diol packing replacing silica gel improves separation stability while ensuring excellent separation results

 

Contact us

 

Add:No.1 Bingang Road, Jingang Development Zone, Binhai Town, Wenling, Zhejiang Province

E-mial:marketing@acchrom.com

Tel:4009653365

Phone:(East)13326073588

              (West) 13306591288

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